A clamp-like biohybrid catalyst for DNA oxidation was written by van Dongen, Stijn F. M.;Clerx, Joost;Norgaard, Kasper;Bloemberg, Tom G.;Cornelissen, Jeroen J. L. M.;Trakselis, Michael A.;Nelson, Scott W.;Benkovic, Stephen J.;Rowan, Alan E.;Nolte, Roeland J. M.. And the article was included in Nature Chemistry in 2013.Recommanded Product: 57412-08-5 The following contents are mentioned in the article:
In processive catalysis, a catalyst binds to a substrate and remains bound as it performs several consecutive reactions, as exemplified by DNA polymerases. Processivity is essential in nature and is often mediated by a clamp-like structure that phys. tethers the catalyst to its (polymeric) template. In the case of the bacteriophage T4 replisome, a dedicated clamp protein acts as a processivity mediator by encircling DNA and subsequently recruiting its polymerase. Here we use this DNA-binding protein to construct a biohybrid catalyst. Conjugation of the clamp protein to a chem. catalyst with sequence-specific oxidation behavior formed a catalytic clamp that can be loaded onto a DNA plasmid. The catalytic activity of the biohybrid catalyst was visualized using a procedure based on an at. force microscopy method that detects and spatially locates oxidized sites in DNA. Varying the exptl. conditions enabled switching between processive and distributive catalysis and influencing the sliding direction of this rotaxane-like catalyst. This study involved multiple reactions and reactants, such as 4-(10,15,20-Tri-p-tolylporphyrin-5-yl)phenol (cas: 57412-08-5Recommanded Product: 57412-08-5).
4-(10,15,20-Tri-p-tolylporphyrin-5-yl)phenol (cas: 57412-08-5) belongs to catalyst ligands. Catalytic transformations have become a mainstay in the toolkit of the synthetic and increasing non-synthetic chemist alike. It is clear that future advancements in metal complexes and their applications crucially depend on ligand design, whereas the ligand electronic, steric and topological properties provide numerous improvements to the reactivity and selectivity at the metal centers.Recommanded Product: 57412-08-5
Referemce:
Metal catalyst and ligand design,
Ligand Template Strategies for Catalyst Encapsulation – NCBI