Allott, Louis published the artcileDetecting hypoxia in vitro using 18F-pretargeted IEDDA “click” chemistry in live cells, Related Products of catalysis-chemistry, the publication is RSC Advances (2021), 11(33), 20335-20341, database is CAplus and MEDLINE.
We have exemplified a pretargeted approach to interrogate hypoxia in live cells using radioactive bioorthogonal inverse electron demand Diels-Alder (IEDDA) “click” chem. Our novel 18F-tetrazine probe ([18F]FB-Tz) and 2-nitroimidazole-based TCO targeting mol. (8) showed statistically significant (P < 0.0001) uptake in hypoxic cells (ca. 90%ID per mg) vs. normoxic cells (<10%ID per mg) in a 60 min incubation of [18F]FB-Tz. This is the first time that an intracellularly targeted small-mol. for IEDDA “click” has been used in conjunction with a radioactive reporter mol. in live cells and may be a useful tool with far-reaching applicability for a variety of applications.
RSC Advances published new progress about 1466420-02-9. 1466420-02-9 belongs to catalysis-chemistry, auxiliary class Copper-Free Click Chemistry,Tetrazine, name is (4-(6-Methyl-1,2,4,5-tetrazin-3-yl)phenyl)methanamine trifluoroacetic acid, and the molecular formula is C12H12F3N5O2, Related Products of catalysis-chemistry.
Referemce:
https://courses.lumenlearning.com/boundless-chemistry/chapter/catalysis/,
Catalysis – Wikipedia