Mukhortava, Ann published the artcileEfficient Formation of Site-Specific Protein-DNA Hybrids Using Copper-Free Click Chemistry, SDS of cas: 1395786-30-7, the publication is Bioconjugate Chemistry (2016), 27(7), 1559-1563, database is CAplus and MEDLINE.
Protein-DNA hybrids have become increasingly popular mol. building blocks in bionanotechnol. and single-mol. studies to synergistically combine the programmability of DNA with the chem. diversity of proteins. The growing demand for protein-DNA hybrids requires powerful strategies for their conjugation. Here, we present an efficient two-step method for protein-DNA assembly based on copper-free click chem. The method allows site-specificity and high coupling efficiency, while maintaining the conservation of protein activity. We compare our method to a commonly used protocol of direct linkage of maleimide-modified oligos. We demonstrate the significantly higher yield with a protein-DNA conjugate, which is analyzed using single-mol. force spectroscopy.
Bioconjugate Chemistry published new progress about 1395786-30-7. 1395786-30-7 belongs to catalysis-chemistry, auxiliary class Inhibitor, name is Dbco-maleimide, and the molecular formula is C25H21N3O4, SDS of cas: 1395786-30-7.
Referemce:
https://courses.lumenlearning.com/boundless-chemistry/chapter/catalysis/,
Catalysis – Wikipedia