Li, Lihong published the artcileSensitive Fluorescence Probe with Long Analytical Wavelengths for ¦Ã-Glutamyl Transpeptidase Detection in Human Serum and Living Cells, Application In Synthesis of 10510-54-0, the publication is Analytical Chemistry (Washington, DC, United States) (2015), 87(16), 8353-8359, database is CAplus and MEDLINE.
A new sensitive fluorescent probe (I) with long anal. wavelengths for ¦Ã-glutamyl transpeptidase (GGT) assay has been developed by incorporating the ¦Ã-glutamyl group as a recognition unit into the fluorophore of cresyl violet (CV). The detection mechanism of I is based on the GGT-catalyzed cleavage of the ¦Ã-glutamyl group, followed by the release of CV, which leads to a distinct color change from light yellow to pink and a large fluorescence enhancement at 615 nm (¦Ëex = 585 nm). Under the optimized conditions, the fluorescence intensity of I is directly proportional to the activity of GGT at 1-50 U/L with a detection limit of 5.6 mU/L. By virtue of its high sensitivity and long anal. wavelengths, I has been used to directly determine GGT in human serum samples from both healthy people and liver cancer patients, and the obtained results accord well with those acquired by com. GGT fluorometric assay kit. I has also been employed to image endogenous GGT in living cells. Notably, with the authors’ probe, the expression level of GGT in HepG2 cells under the action of sodium butyrate (an anticancer drug) was studied by fluorescence confocal microscopy, revealing that sodium butyrate can induce the upregulation of GGT in HepG2 cells in a dose- and time-dependent manner. This behavior of sodium butyrate has further been confirmed by lysate assay and inhibitor experiment I is rather simple and may find a wide use in the determination of GGT in clin. and biol. samples.
Analytical Chemistry (Washington, DC, United States) published new progress about 10510-54-0. 10510-54-0 belongs to catalysis-chemistry, auxiliary class Other Aromatic Heterocyclic,Salt,Amine,Inhibitor,Inhibitor, name is 5,9-Diaminobenzo[a]phenoxazin-7-ium acetate, and the molecular formula is C18H15N3O3, Application In Synthesis of 10510-54-0.
Referemce:
https://courses.lumenlearning.com/boundless-chemistry/chapter/catalysis/,
Catalysis – Wikipedia