Solomon, Michael published the artcileA coupled fluorometric rate assay for pyruvate dehydrogenase in cultured human fibroblasts, Category: catalysis-chemistry, the publication is Analytical Biochemistry (1984), 141(2), 337-43, database is CAplus and MEDLINE.
A method for measuring the activity of the pyruvate dehydrogenase complex (PDC) by coupling acetyl-CoA production to acetylation of a fluorescent dye is described. Acetylation of cresyl violet acetate by pigeon liver acetyltransferase results in a shift of its fluorescence spectrum from ¦Ëex?max = 575, ¦Ëem?max = 620 nm to ¦Ëex?max = 475, ¦Ëem?max = 575 nm (where ¦Ë = wavelength and ex and em = excitation and emission, resp.). The rate of appearance of acetylated dye was followed fluorometrically and was proportional to PDC activity in extracts of cultured human fibroblasts. The assay showed appropriate substrate and cofactor dependence and had a working range between 0.04 and 70 milliunits. It is 10-fold more sensitive than the spectrophotometric assay on which it is based (working range 0.4-31 milliunits) and is equally convenient. Unactivated PDC activity in fibroblast extracts was 0.75 (0.60-0.92) milliunits/mg protein (mean and range for 6 cell lines).
Analytical Biochemistry published new progress about 10510-54-0. 10510-54-0 belongs to catalysis-chemistry, auxiliary class Other Aromatic Heterocyclic,Salt,Amine,Inhibitor,Inhibitor, name is 5,9-Diaminobenzo[a]phenoxazin-7-ium acetate, and the molecular formula is C17H20ClN3, Category: catalysis-chemistry.
Referemce:
https://courses.lumenlearning.com/boundless-chemistry/chapter/catalysis/,
Catalysis – Wikipedia