Hahn, Dongyup published the artcileDetermination of sequence and absolute configuration of peptide amino acids by HPLC-MS/CD-based detection of liberated N-terminus phenylthiohydantoin amino acids, Recommanded Product: (S)-2-Amino-4-(methylthio)butanoic acid, the publication is Scientific Reports (2022), 12(1), 10285, database is CAplus and MEDLINE.
We report a method for the simultaneous determination of the sequence and absolute configuration of peptide amino acids using a combination of Edman degradation and HPLC-MS/CD. Phenylthiohydantoin (PTH) derivatives of 20 pairs of standard D- and L-amino acids were synthesized by the Edman reaction. The CD spectra of the derivatives revealed that each pair of the PTH derivatives exhibited the absorption with opposite signs at around 270 nm. These standard PTH derivatives showed well-resolved resolution without interference from byproducts in the ion chromatogram and clear pos./neg. CD absorptions when subjected on a reversed phase HPLC-MS system coupled with a CD-2095 HPLC detector. This method was applied for the detection of a synthetic pentapeptide and a natural depsipeptide (halicylindramide C). The sequence and configuration of the pentapeptide and up to eight residues of halicylindramide C were successfully analyzed by this method. The amino acid configuration of the pentapeptide was also determined successfully by subjecting its acid hydrolyzates to the Edman reaction followed by HPLC-MS/CD.
Scientific Reports published new progress about 63-68-3. 63-68-3 belongs to catalysis-chemistry, auxiliary class Natural product, name is (S)-2-Amino-4-(methylthio)butanoic acid, and the molecular formula is C5H11NO2S, Recommanded Product: (S)-2-Amino-4-(methylthio)butanoic acid.
Referemce:
https://courses.lumenlearning.com/boundless-chemistry/chapter/catalysis/,
Catalysis – Wikipedia