Schmitz-Moormann, Paul published the artcileTissue staining by basic dyes. I. Influence of the pH of the staining solution and of the dye-affinity on the adsorption of the dye, HPLC of Formula: 10510-54-0, the publication is Histochemie (1968), 16(1), 23-35, database is CAplus and MEDLINE.
Diluted heat-coagulated egg-white, citrated defibrinated beef-serum, and minced beef muscle were washed with alc., acetone, and ether, dried at 50¡ã and ground. Dye adsorption on the protein powders and on Sephadex, G-25 fine, was determined by shaking 1 g. dry protein or Sephadex with 20 ml. buffered 10-4M dye for 1 hr., leaving at room temperature overnight, shaking again, and centrifuging. Michaelis buffers, ionic strength 0.1, 0.1 N HCl, and N HCl were used. Methylene blue, toluidine, blue, cresyl fast violet, crystal violet, alcain blue, thioflavine T, and acridine orange were tested. Except for Sephadex, which failed to stain with methylene blue, the 3 protein powders stained minimally at pH 1 and staining increased to a maximum at pH 9. Swelling of proteins was maximal at pH 1, but varied little with pH above pH 2. A chromatog. method was developed in which 10 g. protein or Sephadex was allowed to swell in water overnight, transferred to a chromatographic column, and washed with buffer. Dye (0.01 M) diluted with 1 volume buffer was applied and the column developed with buffer. The fraction (25 ml.) in which the peak dye concentration appeared varied with pH. Plots of the number of the peak fractions against pH gave a measure of the affinity of various dyes. All dyes were retarded with increasing pH. The order of affinity was crystal violet > acridine orange > toluidine blue > methylene blue ¡Ö thioflavine T. With Sephadex there was no dependence on pH and the order of dye affinity was reversed.
Histochemie published new progress about 10510-54-0. 10510-54-0 belongs to catalysis-chemistry, auxiliary class Other Aromatic Heterocyclic,Salt,Amine,Inhibitor,Inhibitor, name is 5,9-Diaminobenzo[a]phenoxazin-7-ium acetate, and the molecular formula is C18H15N3O3, HPLC of Formula: 10510-54-0.
Referemce:
https://courses.lumenlearning.com/boundless-chemistry/chapter/catalysis/,
Catalysis – Wikipedia