Heydari, Paniz published the artcileDevelopment and validation of an LC-MS/MS assay for the quantification of cintirorgon (LYC-55716) in mouse plasma and tissue homogenates, Quality Control of 2055536-64-4, the publication is Journal of Pharmaceutical and Biomedical Analysis (2022), 114421, database is CAplus and MEDLINE.
Cintirorgon (LYC-55716) is a promising first-in-class antitumor agent as a ROR¦Ã agonist in the treatment against various types of cancer. To support preclin. mouse studies, a bioanal. method was developed and successfully applied for quantification of cintirorgon in mouse plasma and tissue homogenates using LC-MS/MS. The method was fully validated in mouse plasma and partial validation was performed in eight different homogenates originating from brain, kidney, liver, lung, small intestine, small intestine content, spleen, and testis. Sample preparation was performed using 96-well plates for fast and efficient anal. Protein precipitation was done by addition of 20¦ÌL acetonitrile containing monensin as internal standard to 10¦ÌL sample. Chromatog. separation was achieved on a Polaris 3 C18-A column using gradient elution with 0.2% (volume/volume) formic acid and 0.2% (volume/volume) ammonium hydroxide in water (A) and methanol (B) as eluents. The total run time was 3 min. Detection was carried out with a triple quadrupole mass spectrometer with electrospray ionization operated in the pos. ion-mode. Quantification could be accomplished within a linear validated concentration range of 5-4,000 ng/mL (10-4,000 ng/mL in brain homogenates) with an intra- and inter-day precision between 4.6-14.7% and 5.1-15.6% (including the LLOQ), resp., and accuracies between 89.1%-111.2%. The method was successfully applied to a preclin. study with cintirorgon in mice.
Journal of Pharmaceutical and Biomedical Analysis published new progress about 2055536-64-4. 2055536-64-4 belongs to catalysis-chemistry, auxiliary class Metabolic Enzyme,ROR, name is (S)-3-(6-(3-(Difluoromethoxy)-5-fluorophenyl)-4-((3-(trifluoromethyl)phenyl)sulfonyl)-3,4-dihydro-2H-benzo[b][1,4]oxazin-2-yl)-2,2-dimethylpropanoic acid, and the molecular formula is C27H23F6NO6S, Quality Control of 2055536-64-4.
Referemce:
https://courses.lumenlearning.com/boundless-chemistry/chapter/catalysis/,
Catalysis – Wikipedia