Fu, Hongxia published the artcileRatiometric Fluorescence Azide-Alkyne Cycloaddition for Live Mammalian Cell Imaging, Recommanded Product: 5,9-Diaminobenzo[a]phenoxazin-7-ium acetate, the publication is Analytical Chemistry (Washington, DC, United States) (2015), 87(22), 11332-11336, database is CAplus and MEDLINE.
Click chem. with metabolic labeling has been widely used for selectively imaging biomacromols. in cells. The first example of azide-alkyne cycloaddition for ratiometric fluorescent imaging of live cells is reported. The precursor of the azido fluorophore (cresyl violet) has a fluorescence emission peak at 620 nm. The electron-rich nitrogen of the azido group blue-shifts the emission peak to 566 nm. When the click reaction occurs, an emission peak appears at 620 nm due to the lower electronic d. of the newly formed triazole ring, which allows the authors to ratiometrically record fluorescence signals. This emission shift was applied to ratiometric imaging of propargylcholine- and dibenzocyclooctyne-labeled human breast cancer cells MCF-7 under laser confocal microscopy. Two typical triazole compounds were isolated for photophys. parameter measurements. The emission spectra presented a fluorescence emission peak around 620 nm for both click products. The results further confirmed the emission wavelength change was the result of azide-alkyne cycloaddition reaction. Since nearly all biomols. can be metabolically labeled by reported alkyne-functionalized derivatives of native metabolites, the authors’ method can be readily applied to image these biomacromols.
Analytical Chemistry (Washington, DC, United States) published new progress about 10510-54-0. 10510-54-0 belongs to catalysis-chemistry, auxiliary class Other Aromatic Heterocyclic,Salt,Amine,Inhibitor,Inhibitor, name is 5,9-Diaminobenzo[a]phenoxazin-7-ium acetate, and the molecular formula is C18H15N3O3, Recommanded Product: 5,9-Diaminobenzo[a]phenoxazin-7-ium acetate.
Referemce:
https://courses.lumenlearning.com/boundless-chemistry/chapter/catalysis/,
Catalysis – Wikipedia