Zhang, Zeyu published the artcileExplant disinfection with chlorine dioxide promotes adventitious shoot organogenesis in Crassula portulacea via the regulation of reactive oxygen species and related gene expression, SDS of cas: 6972-05-0, the publication is Plant Cell, Tissue and Organ Culture (2021), 146(3), 531-540, database is CAplus.
Abiotic oxidative stress may activate plant organ development and growth. However, this effect is rarely reported for in vitro tissue culture. We report enhanced adventitious shoot formation in Crassula portulacea through the disinfection of explants with an environmentally safe oxidant chlorine dioxide (CD) and explore the involvement of reactive oxygen species (ROS) accumulation and the expression of organogenesis-associated genes. A concentration of 0.62-1.24 mM CD was effective at disinfecting C. portulacea explants within 15 min. Interestingly, the disinfection of explants with CD significantly promoted the formation of adventitious shoots, resulting in a 3-5-fold enhancement of the number of adventitious shoots when compared with the disinfection of explants using 0.1% mercuric chloride. Superoxide anions, hydrogen peroxide and superoxide dismutase increased in the CD treatment, while the enhancements were significantly suppressed when N,N’-dimethylthiourea (DMTU) was applied, indicating that CD-promoted adventitious shoot formation correlates with ROS accumulation. The expression of CpWUS, CpCYCB1 and CpCUC1 was upregulated in explants disinfected with CD, while the application of DMTU significantly suppressed their expression, consistent with adventitious shoot development. These findings present new insights into understanding the physiol. and mol. mechanisms of plant tissue culture and providing an efficient micropropagation protocol for C. portulacea.
Plant Cell, Tissue and Organ Culture published new progress about 6972-05-0. 6972-05-0 belongs to catalysis-chemistry, auxiliary class Thiourea,Amine,Aliphatic hydrocarbon chain,Amide, name is 1,1-Dimethylthiourea, and the molecular formula is C13H9FO2, SDS of cas: 6972-05-0.
Referemce:
https://courses.lumenlearning.com/boundless-chemistry/chapter/catalysis/,
Catalysis – Wikipedia