Zheng, Anqi published the artcileA binding-enhanced but enzymatic activity-eliminated human ACE2 efficiently neutralizes SARS-CoV-2 variants, SDS of cas: 63-68-3, the publication is Signal Transduction and Targeted Therapy (2022), 7(1), 10, database is CAplus and MEDLINE.
The goal is to generate a recombinant ACE2 protein that is long lasting without enzymic activity and can potently neutralize different SARS-CoV-2 variants. To screen efficient ACE2 fusion proteins with neutralization activity against both the wild-type SARS-CoV-2 strain (SARS-CoV-2 WT) and variants, we designed a panel of hACE2 proteins with mutations in the binding interface with the SARS-CoV-2 RBD. We focused on five potentially critical hydrophobic or charged residues, T27, D30, K31, H34, and M82, and designed seven mutants (T27F, D30E, K31R, H34F, H34W, M82F, and M82W) that enhance their hydrophobicity or electrification. We found that the binding affinities of two hACE2-mFc mutants, T27F and D30E, were enhanced, the affinities of two others, K31R and H34F, were diminished, and the affinities of the remaining three, H34W, M82F, and M82W, were nearly identical to that of wild-type hACE2 (hACE2-WT). HACE2-WT and mutant proteins can neutralize both SARS-CoV-2 WT and variants infection with decreased the half maximal inhibitory concentrations in Vero cells. Our binding and neutralization analyses indicate that hACE2-T27F-R273Q has potential as a broad antiviral therapeutic against current and future SARS-CoV-2 variants, and provides insights into its potential for the treatment of infections of other SARS-like coronavirus who use the ACE2 as entry receptor.
Signal Transduction and Targeted Therapy published new progress about 63-68-3. 63-68-3 belongs to catalysis-chemistry, auxiliary class Natural product, name is (S)-2-Amino-4-(methylthio)butanoic acid, and the molecular formula is C5H5NO3S, SDS of cas: 63-68-3.
Referemce:
https://courses.lumenlearning.com/boundless-chemistry/chapter/catalysis/,
Catalysis – Wikipedia