Yi, Xin published the artcileLigands binding and molecular simulation: the potential investigation of a biosensor based on an insect odorant binding protein, Application In Synthesis of 30670-30-5, the publication is International Journal of Biological Sciences (2015), 11(1), 75-87, 13 pp., database is CAplus and MEDLINE.
Based on mimicking biol. olfaction, biosensors have been applied for the detection of various ligands in complex environment, which could represent one of the most promising research fields. In this study, the basic characters of one insect odorant binding protein (OBP) as a biosensor were explored. To explore the mol. recognition process, the tertiary structure of the protein was modeled and the protein-ligand interactions with 1,536,550 chems. were investigated by the mol. docking. The availability of large amount of recombinant SlitOBP1 overcame the difficulty to obtain biol. sensing material. After obtained the purified recombinant protein, the result of fluorescence binding assays proved the candidate protein has good affinities with the majority of the tested chems. With the aid of simulation docking, the key conserved amino acids within the binding site were identified and then mutated to alanine. After mutation, the protein-ligand binding characteristics were recorded, and the competitive binding assays were carried out to provide exptl. verification. The detailed information on its structure and affinities investigated in this study could allow the design of specific mutants with desired characteristics, which provides a solid base for tailoring OBP for biosensor and provides a role model for screening the other elements in olfactory system for different applications.
International Journal of Biological Sciences published new progress about 30670-30-5. 30670-30-5 belongs to catalysis-chemistry, auxiliary class Polyfluoroalkanes, name is 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-Heptadecafluorodecan-1-amine, and the molecular formula is C15H10O2, Application In Synthesis of 30670-30-5.
Referemce:
https://courses.lumenlearning.com/boundless-chemistry/chapter/catalysis/,
Catalysis – Wikipedia